WebIP: Flag HA Flag WCL HA Flag Tubulin j - + MG132 T98G LATS1 Tubulin - + P21 KDa 55 25 130 KDa 55 70 130 KDa 55 70 130 KDa 55 70 130 KDa 55 70 130 40 130 55 KDa KDa 55 40 130 KDa 55 40 130 ... IB analysis of WCLs and immunoprecipitates (IPs) derived from 293T cells transfected with indicated constructs. Cells were treated with MG132 (10μM ... WebApr 12, 2014 · IB是免疫印记、IP是免疫共沉淀、Flag和HA都是一种蛋白标签. 从最右边看，表示细胞再转染了这三种质粒后，能够在细胞中表达，最左边的图是用带有Flag标签的VEGFR拉p85及其研究片段，从图中可以看出，p85被拉出，而其研究片段没有被拉出，中间的图是反过来用p85 ...
Co-IP的结果解读 - 知乎 - 知乎专栏
WebGFP IP, SHP1 IB: GFP IP, SHP1 IB: GFP IP, SHP2 IB: GFP IP, SHP2 IB: WCL, GAPDH IB: WCL, GAPDH IB: Figure 5B Figure 5-figure supplement 1B-100 kD-75 kD WebMay 29, 2024 · WCL的全称是wholecelllysate,中文就是全细胞裂解液对吧?如果是这样的话,这个部分的目的就是研究细胞里面你想要研究的蛋白表达情况.IP的就是你用抗体pulldown … leather wingback chair pottery barn
LATS1 is a central signal transmitter for achieving full type-I ...
Web先看一下图中的缩写： IP全称是immunoprecipitation，即免疫沉淀，这一步主要是为了纯化目的蛋白。 IB全称是immunoblotting，即免疫印迹，也就是常规的Western Blotting，用 … WebWhy American University is the Best Place to Study IP. American University Washington College of Law is perennially recognized as among the top places in the world to study intellectual property law. The program builds on AUWCL's other major strengths, including international law, public interest law and clinical legal education. WebMar 31, 2024 · Immunoglobulin G (IgG) served as a negative control. (D) IB analysis of the WCL and anti-LAMTOR1 IP derived from 5637 cells. IgG served as a negative control. (E) IB analysis of the WCL and anti-HA IP derived from the 293T cells transfected with Flag-NMT1 and HA-LAMTOR1. (F) Detection of myristoylated LAMTOR1 after knocking down of … how to draw a rolls royce car step by step