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Biotinylated mouse tim4-fc

WebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT … WebRegarding Tim4 expression, cells were stained with PE-labeled cIg, or RMT4-54 (anti-Tim4: BioLegend). Mouse peritoneal resident cells were pretreated with clone 93 (anti-CD16/32: BioLegend) to block Fc receptors, and then stained with FITC-anti-CD11b (BioLegend) and the above mAbs. These cells were analyzed by an Accuri C6 cytometer (BD ...

Tim-4 (mouse):Fc (human) (recombinant protein) - Order from …

WebMouse Ig cocktail. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. WebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with … ray\\u0027s hunting lodge newfoundland https://camocrafting.com

Anti-mouse IgG (H+L), Biotinylated Antibody #14709

WebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express … WebTim4 alone did not support efferocytosis, but enhanced TAM-dependent efferocytosis. Resident peritoneal macrophages, Kupffer cells, and CD169+ skin macrophages … WebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ... ray\u0027s hunting and fishing

TIM-4 is the ligand for TIM-1, and the TIM-1–TIM-4 interaction ...

Category:Mouse TIM4/Human Fc Chimera, recombinant

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Biotinylated mouse tim4-fc

Tim-4 (mouse):Fc (human) (recombinant protein) - Order from …

WebTIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by … WebApr 9, 2024 · Moderate. Difficult. Very difficult. Pronunciation of biotinylated with 1 audio pronunciations. 1 rating.

Biotinylated mouse tim4-fc

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WebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … WebOct 24, 2007 · Mouse Tim4 is a type I membrane protein, consisting of a signal sequence and extracellular, ... Tim4-Fc-coated latex beads incubated with Tim1-Fc in the absence …

WebJun 29, 2024 · An ELISA plate (AGC Techno Glass, Shizuoka, Japan) was coated with 1 μg/mL recombinant mouse TIM4-Fc protein (FUJIFILM Wako Pure Chemical)/50 mM carbonate-bicarbonate buffer (pH 9.6) and ... WebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these …

WebOutline: This product is for research use only. Do not administer it to human. This is a recombinant fusion protein; the extracellular domain of mouse TIM4 (TIMD4) is linked to the N-terminus of human IgG 1 Fc domain … WebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg ...

WebThis antibody was developed for use in flow cytometry. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with …

WebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. ray\\u0027s hutfotografieWebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This … ray\\u0027s hydraulicsWebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … ray\u0027s huli huli chicken recipeWebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … simply red hop farm 2022WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 … ray\\u0027s hvac schaumburgWebFeb 9, 2024 · Tim4 is involved in recognition of MWCNTs by mouse peritoneal macrophages. (A) Recognition of MWCNTs (30 μg/mL) by WT or Tim4−/− mouse … ray\u0027s hvac schaumburg ilWebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM … ray\\u0027s hydraulic service llc